Elasmobranch physiology differs significantly from that of teleosts and must be considered when planning and executing elasmobranch transport. Many stressors, physical, sensory, and chemical, occur during elasmobranch transport, resulting in morbidity, mortality, and shortened longevity. Mitigation of losses requires consideration of species biology, anatomy, and physiology. Trauma can be reduced by considering anatomy and operational practices. Physiological stress can be reduced by attention to vibrational and light stressors—maintaining appropriate dissolved oxygen, pH, and temperature supports homeostasis. Reduction of urea toxicity, hypoxia, acidosis, ionic imbalance, and hypoglycemia encourages success. Planning, practice, execution, and post-transport evaluation are necessary for improvement, increased elasmobranch survival, and longevity.