Introduction: Cardiac troponin I (cTnI) Is a biomarker of myocardial damage. Increased circulating cTnI occurs with diseases such as myocarditis and systemic inflammation in other species. Previously a point of care assay measuring cTnI has been validated in rabbits and a reference interval established.1 Methods: Whole blood cTnI was measured in client-owned rabbits. Patient specific variables such as vitals, biochemistry, hematology results, 7-day survival, and echocardiograms were also collected if available. Rabbits were sorted into four major health status categories: apparently healthy (AP), cardiovascular disease (CVD), non-cardiovascular disease (NCVD), and both cardiac and non-cardiovascular disease (BT). Results: In total, 92 rabbits were enrolled (cTnI median 0.035 ng/ml, range 0-37.82 ng/mL). Of those, 40 (43.5%) rabbits had cTnI greater than the reference range (<0.04 ng/mL). There was a positive correlation between cTnI and BUN (Sperman’s p 0.47, p <0.001). While not statistically significant, the median cTnI was higher in CVD (0.092, range 0.01-2.49) and BT (0.075, range 0.01-37.82) categories compared to AP (0.02, range 0-0.09). Higher cTnI was associated with survival in a general linear model. Rabbits that died had a median cTnI 26.75 times those that survived (95% CI 6.36-11151). When survival was removed from this model, both temperature (p=0.039) and BUN (p=0.002) were positively associated with cTnI. Conclusions: Myocardial injury likely occurs in rabbits with both cardiovascular disease and non-cardiovascular disease. Evaluation of the myocardium and an echocardiogram may be warranted in ill rabbits. While further studies are needed, survival was lower in rabbits with higher cTnI concentrations.