A 4-year-old, male neutered, domestic ferret presented with a peracute onset of neurologic symptoms: dull mentation, circling to the left, and a right-sided proprioceptive ataxia. No historic illnesses were reported, and no other significant abnormalities were found on physical examination. Whole-body radiographs, hematology, and biochemistry findings were unremarkable. The ferret was initially treated with supportive care and corticosteroids (prednisolone). Magnetic resonance imaging (MRI) with diffusion-weighted imaging (DWI) demonstrated a restricted diffusion in the left cerebral artery with a focal edematous pattern within the left cerebrum consistent with a left middle cerebral artery occlusion. Corticosteroids (anti-inflammatory dose) were continued until clinical symptoms were resolved. A 2-month follow-up MRI demonstrated unrestricted diffusion in the region of the left cerebral artery and resolving cerebral edema. The findings and clinical signs were consistent with an ischemic infarct in the left cerebrum. To our knowledge, this is the first report of antemortem diagnosis of a CVA in a ferret. In cats and dogs, ischemic stroke occurs most commonly in the cerebellum, with only one report each of cerebral infarction in a cat and a dog. This case demonstrates an important differential to consider in a ferret exhibiting acute central neurologic symptoms.