Evaluating blood glucose quickly and accurately in ferrets is essential not only in emergency situations but also for the effective long-term management of insulinoma. Historically, clinicians relied—within reasonable limits—on AlphaTrak 2 results despite the absence of a ferret-specific calibration. With the transition to the AlphaTrak 3, uncertainty has arisen regarding which species setting is most appropriate and how accurate the device is for ferret patients. Two private practice animal hospitals collected samples from more than 70 normoglycemic and hypoglycemic ferrets to compare blood glucose measurements obtained using the AlphaTrak 3 (both Cat and Dog settings) and the Vetscan VS2. Plasma from most samples was subsequently submitted to a university laboratory for additional comparison using an i-STAT analyzer and a standard wet chemistry analyzer. The objectives were to (1) assess the reliability of the AlphaTrak 3 for ferret blood glucose evaluation and (2) evaluate i-STAT accuracy, given that its single-analyte glucose cartridges are more cost-effective than Vetscan rotors. Initial in-clinic testing demonstrated considerable variability in AlphaTrak 3 readings. Neither the Cat nor Dog setting consistently produced higher values, and discrepancies of up to 30 mg/dL were observed between settings, indicating inconsistent performance. When compared to Vetscan VS2 results, AlphaTrak 3 readings were generally lower or closely aligned, suggesting that the Vetscan VS2 may provide more reliable measurements. Data from the i-STAT and chemistry analyzer are still being processed, and final statistical analyses are pending.