Squamous cell carcinoma (SCC) is an uncommonly reported neoplasia in tortoises, especially of the distal limbs. In dogs and cats, these are typically associated with the skin, oral cavity, and digits (Webb et al. 2009). Reports of SCC in tortoises have found primary lesions in the carapace and plastron (von Deetzen et al. 2012) as well as the facial region (Iannoccone et al. 2011), but no other reports have documented it arising from the distal limb. In this case, surgical removal via amputation of the lower hind limb is described with associated histopathology results that ultimately allowed for diagnosis of this disease in a 20 year old, female Leopard tortoise (Stigmochelys pardalis). Additionally, initial lesions were first noted by the owner approximately 1 year prior to presentation, suggesting a relatively slow progression of this neoplasia but with no current clinical evidence of metastasis.