Performing safe, effective anesthesia can be challenging in amphibians given their unique physiology. Anticholinergics may be warranted to counteract anesthesia-related bradycardia. However, information concerning the clinical use of anticholinergics in amphibians is limited. The objective of this randomized crossover study was to evaluate the effects of atropine and glycopyrrolate on Sonoran Desert toads (Incilius alvarius). In Part 1, 19 toads received intramuscular atropine 0.1 mg/kg, glycopyrrolate 0.025 mg/kg (LG), glycopyrrolate 0.05 mg/kg (HG), saline, or negative control. Heart rate (HR) was monitored at baseline and 1, 3, 5, 10, 15, 20, 25, 30, 40, 50, and 60 minutes. In Part 2, 10 toads were given intramuscular alfaxalone 10 mg/kg followed by glycopyrrolate 0.05 mg/kg or saline. HR was monitored at baseline and 1, 3, 5, 10, 15, 20, 25, 30, 40, 50, 60, 80, 100, and 120 minutes. In Part 1, all three protocols significantly increased the HR between 3 and 30 minutes vs. both controls, except for LG and HG vs. saline at 20 minutes and LG vs. both controls at 30 minutes. HR was significantly increased by LG at 40 minutes (vs. saline); LG and HG at 50 minutes (vs. negative); and LG and HG at 60 minutes (vs. saline). In Part 2, glycopyrrolate significantly increased the HR vs. saline at 10 minutes. Atropine and glycopyrrolate significantly increased the HR in conscious toads, but the effective period of glycopyrrolate was briefer in sedated toads. Additional studies are indicated to further evaluate the clinical efficacy of anticholinergics in amphibians.