The African rock python (Python natalensis) is a protected species in Zimbabwe, but the diseases affecting this giant snake are not well understood. This study aimed to identify endoparasites and haemoparasites in African rock pythons at selected sites in Zimbabwe. Thirty pythons were captured, and blood and faecal samples were collected before the snakes were released on-site. A higher number of male snakes were observed than females. Peripheral blood smears were prepared, air-dried, stained with Giemsa stain, and examined under a light microscope at ×100 magnification. Thirteen faecal samples were analysed for parasite eggs using flotation and sedimentation techniques. Only one haemoparasite, Hepatozoon spp. was identified, with a prevalence of 66% (20/30) in the sampled population. Severe parasitaemia was noted in snakes longer than 200 cm, likely due to increased exposure to vectors. Most snakes exhibited moderate infections of Hepatozoon spp., which is known to cause severe anaemia and may predispose individuals to additional infections. Three nematode endoparasite species, including Oxyuris, Kalicephalus, and Ophidascaris, were identified in faecal samples, with Oxyuris being the most prevalent. One snake had co-infections with Kalicephalus and Oxyuris spp. Both types of parasites can lead to gastrointestinal issues such as obstruction and gastritis, as well as reduced reproductive potential in pythons. Understanding these parasitic infections is crucial for informing conservation strategies and health management for this vulnerable species in captive situations.