Per- and polyfluoroalkyl substances (PFAS) are ubiquitous in the environment, particularly in water, and detectable in nearly all screened human blood in developed countries. PFAS detection protocols have been standardized for different sample types by the Environmental Protection Agency (EPA) and Centers for Disease Control and Prevention (CDC). EPA Method 1633 (EPAM) is used for aqueous, tissue, and biosolid samples. CDC Method 6304.09 (CDCM) is used for serum or plasma samples. Common snapping turtles (Chelydra serpentina) are an aquatic species likely to have detectable PFAS levels. The purpose of this study was to compare PFAS detection using EPAM and CDCM in common snapping turtle plasma. Plasma from 10 common snapping turtles in Illinois, USA was collected in accordance with University of Illinois’ Animal Care and Use Protocol 23206. Screening and quantification of 40 PFAS species was performed using EPAM and CDCM via high performance liquid chromatography-tandem mass spectrometry. CDCM was modified slightly, removing online solid phase extraction. Descriptive statistics were performed. All samples were positive for at least 3 PFAS species and all were positive for perfluorodecanoic acid and perfluorooctanesulfonic acid. More PFAS species were detected per sample using EPAM (median 6 [range 3-9]) compared to CDCM (4 [3-5]). A single species, perfluorobutanoic acid, was detected in more samples using CDCM than EPAM (7 and 4, respectively). Further analysis is ongoing. Although EPAM is not typically used for plasma, it appears to be more sensitive than CDCM for detection of certain PFAS species in common snapping turtle plasma.