Batrachochytrium dendrobatidis (Bd), B. salamandrivorans (Bsal), ranaviruses, bufonid herpesvirus 1 (bfHV1), and ranid herpesvirus 3 (RaHV3) have all been found in various amphibian species and in various locations in Germany. However, screening for these pathogens in wild amphibians has been sporadic. In 2023, a project screening samples from 310 amphibians from seven different species in the state of Brandenburg for all of these pathogens by PCR detected Bd in 8.71% of the samples, including 45.83% of the European fire-bellied toads (Bombina bombina) tested. BfHV1 was found in 33.23% of the samples, including in 90.53% of the common toads (Bufo bufo) and 10.53% of the northern crested newts (Triturus cristatus) tested. RaHV3 was found in 0.65% of the samples, in a water frog (Pelophylax spp.) and a northern crested newt. A follow-up study was carried out in 2024 testing 92 animals and focusing on European fire-bellied toads, common toads, and northern crested newts. Bd was not found in any of the animals tested (P < 0.0001) and BfHV1 was also found at a significantly (P < 0.0001) lower prevalence (54.84%) in common toads in 2024. RaHV3 was found in common toads, northern crested newts, and European fire-bellied toads in 2024 and prevalence of RaHV3 was significantly (P < 0.0001) higher in 2024 (12.9%) than in 2023 (0.65%). Additional testing is planned in 2025 to better understand the epidemiology of these pathogens in amphibians in this area and their impact on these amphibian populations, which are in decline.