Leopard geckos (Eublepharis macularius) are a common species in managed care, and coeliotomies are performed for a wide variety of reasons. As in other species, dehiscence can occur as a surgical complication. Medical records from four institutions over 15 years (2009–2024) were reviewed, and nine adult leopard geckos (2 males and 7 females of ages ranging from 1 to 8 years) with incisional dehiscence were identified. Ventral paramedian coeliotomies were performed under general anesthesia for gastrointestinal foreign bodies (n = 2), dystocia (n = 5), and hepatic biopsies (n = 2). A variety of suture patterns and monofilament suture were used for closure, and both single and double layer closures were used. Body wall incisional dehiscence occurred 1-14 days post-operatively (mean 7 days). Two geckos were euthanized after dehiscence prior to any additional surgical intervention. Three geckos recovered uneventfully after one revision surgery, and four geckos underwent multiple surgical revisions and were ultimately euthanized. Three geckos underwent a full necropsy, and two had myositis of the body wall suggesting inflammation of the muscular layer as a cause of the dehiscence, though no infectious agents were noted. Leopard geckos may have an increased risk of coeliotomy dehiscence, and small patient size and thin body wall thickness compared to other lizard species may be contributing factors. Further research is needed to investigate the histology of the ventral coelom in this species to determine if different surgical techniques for coeliotomy closure are warranted, as has previously been conducted in fish.