Understanding the post-prandial changes associated with different diets may provide insights into dietary recommendations and pre-analytical considerations for biochemistry interpretation. The objectives of this study were to evaluate the effect of fasting duration and diet types (plant-based and crickets) on plasma glucose, uric acid, cholesterol, triglycerides, non-esterified fatty acids (NEFA), β-hydroxybutyric acid (BHBA), and bile acids after a single feeding. Hepatic fat content was estimated on CT-scan. Twenty-two bearded dragons were randomly divided into 2 groups, each receiving either a plant-based or cricket diet. Following a 72h fasting period, animals were gavage-fed their respective diets. Blood samples were collected at 0, 6, 12, 24, 48 and 72h post-feeding for biochemistry analysis. Data were analyzed using linear mixed models. Plasma glucose, uric acid, bile acids, NEFA, and BHBA concentrations varied significantly over time between the two diets. Cholesterol and triglycerides remained stable throughout the fasting period. Glucose peaked for 48h in the plant-based group. Uric acid increased in the cricket group but declined in the plant-based group. Bile acids increased in both groups, with a more pronounced increase in the cricket group. NEFA and BHBA decreased within the first 12h in both groups, with a subsequent rebound, except for BHBA in the plant-based group. Hepatic CT attenuation significantly influenced multiple analytes. A fasting period of 48-72h depending on analytes is recommended prior to biochemistry analysis as shorter fasting periods may lead to erroneous interpretation of plasma biochemistry. The findings of this study suggest a feeding frequency of 2-3 days for adults.