Axolotls are popular pets and widely used in research, safe anesthetic protocols are needed. The objective of this study was to determine the minimum anesthetic concentration (MAC) of alfaxalone and propofol via immersion and to compare their effects on cardiorespiratory parameters and anesthesia milestones. In a crossover design, 10 adult axolotls (5 females, 5 males) were anesthetized with propofol or alfaxalone. Propofol started at 2 mg/L, and alfaxalone at 8 mg/L. In both protocols, an increment of 1 mg/L every 20 minutes was then added to the water until a complete loss of the righting reflex for 20 seconds was observed. Anesthesia was monitored every 5 minutes, including heart rate, gill movement, anesthetic depth (leg withdrawal, escape response, righting reflex), and anesthetic milestones. Recovery occurred in anesthetic-free water. The doses achieving 50% and 95% probability of righting reflex loss were calculated using mixed logistic regression with dose and sex as fixed effects and individual axolotls as a random effect. Both protocols were well-tolerated. Alfaxalone MAC differed significantly by sex (p<0.001), at 9.5 mg/L for females and 12.5 mg/L for males. Propofol MAC was similar between sexes (p=0.21), averaging 3.0 mg/L. The mean time to loss of the righting reflex was 53 ± 9 minutes for propofol and 82 ± 25 minutes for alfaxalone. Recovery times were 19 ± 5 minutes for propofol and 14 ± 4 minutes for alfaxalone. Both protocols induced a loss of righting reflex in most animals at the MAC dose, supporting their use in minimally-invasive procedures.