This presentation is dedicated to addressing the challenges associated with the diagnosis and treatment of Encephalitozoon cuniculi infection in rabbits. Despite available literature, an international survey of clinicians revealed gaps and variability in practices, with many relying on anecdotal evidence alongside published data. The masterclass aims to enhance knowledge and standardize approaches by highlighting key gaps in current understanding and clinical application, recent research advancements, and development of practice guidelines. A combination of didactic lectures and interactive discussions will provide attendees with practical insights. Case studies will illustrate real-world scenarios, fostering a collaborative dialogue between presenters and participants. This session is designed to bridge the gap between research and practice, equipping clinicians with tools to improve outcomes in managing this challenging infectious condition.