Locoregional anesthesia is a useful aid for perioperative pain management and is widely described in dogs and cats, but is sparsely described in exotic mammals. The current literature primarily describes cadaveric studies or blind techniques, which often result in variable rate of success. This masterclass aims to address this gap by focusing on ultrasound-guided and nerve stimulation-guided locoregional techniques in small mammals, exploring the feasibility, benefits and potential complications of these approaches. In particular, one of the key learning objectives is to provide a practical guidance on implementing regional anesthesia in a clinical setting, focusing on equipment required and accessibility. Moreover, the masterclass will include also clinical cases where the techniques resulted effective, in order to show and emphasize their efficacy and practical application for pain control. Examples of described technique include locoregional anesthesia of thoracic and pelvic limb, head (ear and oral cavity), thoracic and abdominal cavity. Specifically, the covered species include rabbit, guinea pig, chinchilla, hedgehog and others. The use of locoregional anesthesia can make a difference in peri-operative pain management reducing the dose of systemic drugs for anesthesia and providing pain relief during the procedures and during recovery.