Gastrointestinal disease is a common presenting complaint of both adult sugar gliders and joeys, with a recent retrospective finding GI disease as one of the top causes of adult sugar glider morbidity. There is limited evidence about the sugar glider fecal microbiome in both healthy and diseased states. Cultures and PCR testing have been utilized but may not reveal a thorough measurement of all relevant organisms, nor do these methods quantify the amount of bacterial or fungal organisms present. A single study of 12 sugar gliders utilizing genomic sequencing showed that regardless of diet, the most predominant bacteria were shared across individuals. The goal of the current study was to characterize the healthy sugar glider fecal microbiome using next-generation DNA sequencing (NGS) in 54 sugar gliders. The sugar glider microbiome is likely uniquely diverse relative to other exotic companion mammal pets. A better understanding of the normal microbiome will allow for further characterization of potential disease states and pathogens related to the gastrointestinal microflora.