The microbiome of dolphins is one of the most studied among marine mammals, including feces and skin. However, little evidence exists on the blowhole and gastric microbiome. Therefore, the aim of this presentation is to compare the fecal (n=115), gastric (n=69), blowhole (n=52), and skin (n=27) microbiome of 263 dolphins. Next-Generation Sequencing was performed to identify and compare the bacterial and fungal diversity, and particular species that differed between body sites. These findings will emphasize the influence of the environmental microbiome among different body sites in dolphins, highlighting the variances between body sites to provide deeper insights into host-microbe interactions and their implications for dolphin health, disease, and potential treatment