Objective: To characterize cytologic and microbial characteristics of tracheal washes from normal inland bearded dragons (Pogona vitticeps) Animals: 48 adult inland bearded dragons Methods: Tracheal washes were performed under sedation using saline. Recovered fluid was evaluated using cytology and traditional culture methods, including matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Complete blood counts and post-mortem evaluations were performed on all dragons to exclude animals with underlying respiratory disease. Results: 77% of samples grew at least 1 microorganism. The most common isolates were fastidious non-enteric organisms that could not be further identified (48% of samples). Chelonobacter oris and Pseudomonas aeruginosa were also present (14.5 and 10.5% of samples, respectively). Clinical Relevance: Respiratory disease is common in bearded dragons. Sampling of the respiratory tract should be considered as a component of the diagnostic work-up. Presence of microorganisms is normal in a bearded dragon tracheal wash, and antibiotics should not be prescribed based solely on the presence of bacteria. Genomic sequencing may be necessary to further identify the respiratory tract microbiome.