Cardiovascular disease in reptiles is becoming more frequently reported due to availability and utility of advanced diagnostics in nondomestic species. Cardiac troponin I (cTnI) has gained popularity in veterinary medicine as a screening tool for cardiac disease, specifically myocardial injury. This cardiac-specific protein has been previously detected in snake cardiac tissue, however it has not yet been investigated in other reptiles. The aims of this study were to (1) determine if cTnI can be detected with a standard point-of-care (POC) cTnI immunoassay (i-STAT cTnI cartridge, Abbot Point of Care, Abbot Park, IL) in cardiac muscle of lacertilia spp, ophidia spp, and testudine spp, and (2) compare cardiac muscle cTnI concentrations to skeletal muscle and plasma when attainable. Cardiac muscle, skeletal muscle, and plasma were opportunistically collected from reptiles that were euthanized or died naturally at North Carolina State University, North Carolina Zoo, and Center for Marine Sciences and Technology. Tissue samples were processed to create homogenates with determined protein concentrations which were subsequently analyzed on the POC assay. Pilot data from six different species (two lizards, two snakes, two chelonians) revealed detection of cTnI in all cardiac samples (1.67-19.29 ng/dL), and lack of detection in all skeletal samples (0.00 ng/dL). Pilot data from four additional species is pending. Assessment of cTnI detectability in at least 10 different lizard, snake, and chelonian species, for a total of ≥30 species, will strengthen the hypothesis that reptiles have detectable cTnI, providing opportunity for advancements in cardiac diagnostics for class Reptilia.