Amphibians are the vertebrate group most impacted by the current mass extinction. Anthropogenic activities have globally deteriorated the status of amphibians, particularly in the Neotropics. Given the accelerating extinction rate, the Amphibian Conservation Action Plan recommended the establishment of ex-situ survival assurance populations to safeguard those species and for reintroduction programs. Unfortunately, managing these species in captivity can be challenging, especially regarding nutrition. Most commercially available insects are naturally deficient in vitamin A. Therefore, hypovitaminosis A is one of the most common diseases in ex-situ populations of frogs. Frogs raised on vitamin A-deficient diets can develop squamous metaplasia in different organs, impacting growth, the immune system, and breeding success. This experimental study aimed to determine whetherhouse crickets (Acheta domesticus) gut-loaded with different concentrations of vitamin A can transfer this fat-soluble vitamin to 1-year-old red-eye tree frogs (Agalychnis callidryas). The primary hypothesis being tested in the study was that red-eye tree frogs offered high vitamin A gut-loaded crickets (n=9) would have significantly higher liver vitamin A concentrations than frogs fed the low vitamin A diet (n=5). After eight months of being fed gut-loaded crickets with a low or high vitamin A diet, surgical liver biopsies were collected from the 14 red-eye tree frogs. Vitamin A was measured as the total retinol in the liver samples using liquid chromatography–mass spectrometry. Samples are being analyzed and data will be available by 12/21/23. This study represents the first attempt to measure vitamin A concentrations in ante-mortem red-eye tree frogs using liver biopsies.