Over 15 years, seven red-eared sliders (Trachemys scripta elegans) ranging in age from 4-32 years of both sexes from several private and zoologic collections were diagnosed with squamous cell carcinoma via biopsy or necropsy. In at least one case, the neoplasm was the cause of the patient’s euthanasia. The neoplasms presented as mass effects (2/7), plaque-like thickenings (3/7), non-healing wounds (3/7), or a combination thereof on the carapace (1/7), head (1/7), neck (3/7), forearm and neck (1/7), or non-specified skin (1). Lesions that presented as masses often had necrotic centers and evidence of a secondary bacterial infection (foul odor). In some cases, the masses were treated with antibiotics, and some initially responded, but all progressed despite therapy. All the masses had histologic features consistent with squamous cell carcinoma, such as anastomosing trabeculae, “keratin pearls”, and desmoplasia. The neoplasms had mitotic counts that ranged from 1-20 mitoses per 2.37 mm2 (ten 400x fields). The high incidence of this neoplasm in individuals of the same species raised concern for an infectious cause, so a nested conventional polymerase chain reaction (PCR) was performed to attempt to detect a novel herpesvirus, which is pending. This report documents a case series of squamous cell carcinoma in red-eared sliders.