Reptiles present unique challenges to the veterinary intervention of euthanasia including varying vascular anatomy and intrinsic myocardial function that allows the heart to continue to beat after cerebral death. Transmucosal pentobarbital has been investigated in turtle when dosed at 100-200mg/kg it is associated with rapid onset of respiratory arrest and general anesthesia followed by cessation of the heart beat at a median of 18 hours. The objective of this study was to evaluate transmucosal pentobarbital in bearded dragons. Six bearded dragons, maintained at room temperature, being euthanized at the end of another project, we were administered pentobarbital 100mg/kg transmucosally at time zero. After 20 minutes and induction of a generalized anesthetized state, a second dose of 400mg/kg was delivered transmucosally. The bearded dragons were serially monitored for HR, RR, movement, and reflexes (palpebral, corneal) and response to nociception (superficial, deep pain) over 24 hours. Death was confirmed by lack of ECG readings after cessation of heart beat detectable by Doppler. Time to loss of heart rate ranged from 120 to 360 minutes after the initial pentobarbital dose and time to death ranged from 240 to 360 minutes, with several lizards exhibiting pulseless electrical activity. Half of the lizards exhibited signs of discomfort at the time of the initial pentobarbital dose and one lizard demonstrated violent, whole-body spasms immediately after administration of the second dose of pentobarbital. Transmucosal pentobarbital is easily delivered and does not require venous access, however, although additional premedications should be considered to reduce patient reactions.