The effect of intravenous fluid bolus on improving hemodynamic parameters in anesthetic patients has recently been questioned. This prospective, randomized, blinded, cross-over experimental study aimed to compare the blood pressure (BP) and heart rate (HR) during an intravenous bolus of three different fluids in euvolemic, isoflurane-anesthetized rabbits. Ten adult New Zealand White rabbits underwent isoflurane anesthesia and received 25 mL/kg of Lactate Ringer’s solution (LR), 2.53 mL/kg of 7.5% hypertonic saline (HS), and 6.25 mL/kg of 6% tetrastarch 130/0.4 (HES) intravenously over 20 minutes on separate occasions. The fluid doses were determined based on the same sodium content of LR and HS and a 4:1 ratio of LR and HES. Oscillometric systolic arterial pressure (SAP), mean arterial pressure (MAP), diastolic arterial pressure (DAP), and HR were measured at baseline, 5, 10, 15, 20, 30, 40, 50, 60, and every 15 min until 180 min post-infusion. Differences in SAP, MAP, DAP, and HR across treatments and time points were evaluated using a linear mixed-effects model and Tukey test for post hoc analysis. Notably, SAP, MAP, and DAP did not significantly increase after the bolus of three different fluids; however, LR and HES had overall significantly higher SAP, MAP, and DAP than HS. The findings highlight the minimal effect of fluid bolus on increasing BP in euvolemic, isoflurane-anesthetized rabbits, even with relatively higher doses of LR and HES than those conventionally used in clinical practice. Further investigation of alternative doses of fluid boluses in euvolemic, anesthetized rabbits is warranted.