A 2-year-old male castrated sugar glider (Petaurus breviceps) was presented to the Oklahoma State University’s zoological medicine service for a chronic history of foul-smelling discharge on the ventrum. A right paracloacal gland impaction was diagnosed on CT. Brown purulent secretions were expressed from the impacted paracloacal gland and collected for culture and susceptibility. Trimethoprim/sulfamethoxazole was administered orally for empirical treatment pending culture and susceptibility results. Aerobic and anaerobic culture revealed Corynebacterium auriscanis, Corynebacterium aurimucosum, Staphylococcus spp., Streptococcus canis, Enterococcus faecalis, Enterococcus casseliflavus, Enterococcus avium, Pseudomonas aeruginosa, and Helococcus kunzii. Susceptibility testing was performed on Staphylococcus psuedintermedius and Pseudomonas intermedius, revealing multidrug resistance for both species. The patient was anesthetized for paracloacal gland lavage and an antibiotic ointment (neomycin, polymyxin B, and bacitracin zinc) was infused into the paracloacal glands to concentrate the antimicrobials locally. The patient’s clinical signs resolved for 3 months before having a recurrence. Local treatment was elected using a different antimicrobial (florfenicol, terbinafine, mometasone furoate). Microbial DNA sequencing was performed on the secretions to further guide local antimicrobial therapy. The pathogens detected were Corynebacterium auriscanis and multidrug resistant Corynebacterium jeikeium; gentamicin solution was infused into the paracloacal glands the following week based on these results. Paracloacal gland lavage and antimicrobial infusions were repeated weekly for a total of 4 treatments. The local inflammation improved with local treatment and the clinical signs resolved on follow-up. Local therapies with lavage and antimicrobial infusion should be considered for treatment of paracloacal gland impaction in sugar gliders.