Peripheral venous access in rabbits may be challenging to achieve in certain situations. According to the 2020 AVMA Guidelines for Euthanasia, intrarenal injection is categorized as an acceptable with conditions method which can only be performed on a sedated and unconscious animal. On average, the renal blood flow contains 20-30% of the resting cardiac output and the intrarenal route utilizes this circulation to deliver drugs systemically. Sodium pentobarbital is a barbituric acid derivative and the most commonly used euthanasia solution used in a variety of species. Its mechanisms cause depression of the central nervous system (CNS), loss of consciousness, anesthetic overdose, and apnea followed by cardiac arrest. Minimal studies using intrarenal administration have been conducted in felines, but there are no current studies measuring the efficacy, efficiency, and validity in rabbits. In a pilot study using 16 rabbits (8 males and 8 females), we evaluated the time to cardio-pulmonary arrest (CPA) arrest following injection of 3 ml of sodium pentobarbital (390 mg/ml) intrarenal (n=8) compared to intravenous (n=8). Intravenous CPA times averaged 9.75 seconds for cardiac and 9.1 seconds for respiratory. Intrarenal CPA times averaged 7.2 minutes for cardiac arrest and 4.6 minutes for respiratory arrest (p=0.0253 and p=0.0862). Overall, information from this pilot study can be used to guide both laboratory and practicing clinicians that are considering preforming this technique. Given the prolonged time to cardiopulmonary arrest compared to intravenous administration, intrarenal euthanasia at the recommended dosages in rabbits may not be appropriate for all clinical scenarios.