Red-eared sliders (Trachemys scripta elegans) are popular zoological companion animals that routinely require chemical immobilization for veterinary diagnostics and care. However, information regarding injectable procedural sedation options is limited in aquatic turtle species. The object of this randomized, blinded, complete cross-over study was to 1) evaluate the efficacy of subcutaneous (SC) tiletamine-zolazepam for sedation and the effect of injection location on several sedation parameters in red-eared sliders and 2) assess if flumazenil administration decreases recovery time with tiletamine-zolazepam sedation in this species. Tiletamine-zolazepam doses of 2.5, 5, 7.5, 10, and 15 mg/kg where evaluated in initial dose-finding pilot studies. Time to sedation onset, respiratory rate, heart rate, forelimb withdrawal reflex, head withdrawal reflex, superficial pain response, jaw tone, cloacal tone, reactivity to jugular venipuncture, and ability to tape limbs in extension for radiograph positioning were assessed to determine sedation level. 10 mg/kg tiletamine-zolazepam was determined as the optimal dose chosen for the final study trials utilizing 9 adult turtles. Study results to be presented.